pyatac
The pyatac command line function contains several subcommands. To run a subcommand, run pyatac subcommand .... The available subcommands are:
- ins
- bias
- vplot
- bias_vplot
- sizes
- pwm
To see available subcommands, you can also run pyatac --help. For each subcommand, options can be viewed as pyatac subcommand --help.
ins
pyatac ins makes a bedgraph file with insertion frequencies for every base.
If a bedfile is provided, insertion track will only span regions in bedfile. Otherwise track will span whole genome.
Output: A tabix-indexed bedgraph file with insertion densities.
bias
pyatac bias computes a bias track.
If a bedfile is provided, bias track will only span regions in bedfile. Otherwise track will span whole genome.
Output: A tabix-indexed bedgraph file with relative Tn5 preferences. These preferences are in log space.
vplot
pyatac vplot generates a vplot around sites in input bed file.
Output: An eps file with vplot image as well as a text file with vplot data.
bias_vplot
pyatac bias_vplot computes the vplot based on the sequence bias and the global fragment size distribution
Output: An eps file with vplot image as well as a text file with vplot data.
sizes
pyatac sizes computes the fragment size distribution
Output: text file with sizes information
pwm
pyatac pwm computes the relative nucleotide frequencies around sites
Output: text file with PWM information
nucleotide
pyatac nucleotide computes the nucleotide frequencies around sites in the input bedfile
Output: text file with frequencies